Inclusion Criteria:
- - Age: 18-65 years old at the time of informed consent.
- - Signed informed consent form, able to adhere to the study visit schedule, and comply
with other requirements of the study as specified in the protocol.
- - Score of 8 or more points on the Hybrid-SLEDAI with at least 6 points from clinical
items and at least one BILAG A or 2 B.
- - If SLE and LN: Active nephritis Class III or IV with or without Class V using the
2018 International Society of Nephrology and Renal Pathology Society (ISN/RPS)
criteria as evidenced on kidney biopsy during screening or within 12 months before
study enrollment.
Per NIH indices, subjects must have at least activity score ≥ 2
and no more than moderate chronicity index. Subjects must have urinary
protein:creatinine ratio (UPCR) ≥ 1.5 g/g or proteinuria ≥ 1.5 g/day, however,
subjects must have ≤7g/ day of proteinuria.
- - For patients with biopsies older than 6 months we will include an adjudication
committee to review clinical data and decide on appropriateness of including the
patient (see section 7.3).
Additionally, all patients with active LN maximally
tolerated doses of renin angiotensin aldosterone system (RAAS) blockade agents,
except for patients with contraindications or intolerance to RAAS.
- - Women of childbearing potential must have a negative serum pregnancy test at
screening and agree to complete abstinence from intercourse from 2 weeks prior to
administration of the first dose of study agent until 1 year after the last dose of
study agents OR consistent and correct use of acceptable birth control (e.g.
levonorgestrel implants, ethinyl estradiol/etonogestrel vaginal ring; injectable
progesterone, intrauterine device [IUD] with failure rate < 1% per year, oral
contraceptives, double barrier method, transdermal contraceptive patch) OR male
partner sterilization with documentation of azoospermia prior to subject's entry
into the study.
- - Male patients must agree to always use a latex or other synthetic condom during any
sexual activity with women of childbearing potential.
Male participants must agree
to continue to use a condom during the intervention period and for at least 1 year
following last treatment with investigational product. Female partners of male
participants should be advised to use a highly effective method of contraception
during treatment period and at least 12 months following treatment.
- - One of the following: positive antinuclear antibodies (ANA) ≥ 1:80 at screening OR
positive anti-dsDNA OR positive anti-Smith (anti-Sm) as determined by the local
laboratory.
- - Refractory SLE defined as having received ≥ 2 prior therapies for SLE (one
immunosuppressant and one biologic/advanced therapy) and had an inadequate response
to therapy despite being on a therapeutic dose for ≥ 90 days.
- - For subjects taking chronic corticosteroids for SLE/LN management, the dose must be
stable for ≥ 14 days before screening and cannot exceed 20 mg prednisone/day or
equivalent at LD start with planned taper to ≤ 5 mg prednisone by the time of LD
start with planned taper to ≤ 5 mg prednisone by the time of the first NKX019
infusion.
- - Negative SARS-CoV-2 test.
Exclusion Criteria:
- - Estimated glomerular filtration rate (eGFR) as calculated by the Chronic Kidney
Disease Epidemiology Collaboration (CKD-EPI) equation of < 45 mL/min/1.73 m2 at
screening.
- - Currently requiring renal dialysis (hemodialysis or peritoneal dialysis) or expected
to require dialysis during the study period.
- - More than 7 g/ day of proteinuria.
- - Previous solid organ or hematopoietic cell transplant or planned transplant within
study treatment period.
- - Congenital or acquired immunodeficiency resulting in severe infection or those
receiving chronic immunoglobulin replacement therapy.
- - Liver disease or dysfunction, including cirrhosis and/or aspartate aminotransferase,
alanine aminotransferase, or bilirubin ≥ 3 times the upper limit of normal.
- - Pulmonary comorbidity including chronic obstructive pulmonary disease or asthma
requiring daily oral steroids or resting hypoxemia (< 90% oxygen saturation via
pulse oximetry) on room air.
- - >10 pack years of smoking.
- - Exacerbation of COPD/asthma requiring systemic steroid therapy within the past 6
months.
- - Bone marrow insufficiency unrelated to active SLE (according to Investigator
judgment) with white blood cell count < 1,500/mm3; hemoglobin levels < 9 g/dL
absolute neutrophil count (ANC) < 1,000/mm3; absolute lymphocyte count
- - Major cardiac disease, abnormalities, or interventions as defined by, but not
limited to:
1.
Uncontrolled angina or unstable life-threatening arrhythmias. 2. History of myocardial infarction within 12 weeks prior to the first dose of
NKX019. 3. Any prior coronary artery bypass graft surgery. 4. ≥ Class III New York Heart Association (NYHA) congestive heart failure (CHF),
significantly decreased ejection fraction (EF ≤ 45%), or severe cardiac
insufficiency. 5. Prolongation of the QT interval corrected for heart rate (QTc) (Fridericia)
interval of > 480 msec. 6. Peripheral artery bypass graft surgery, pulmonary embolism, or other ≥ Grade 2
thrombotic or embolic events within 12 weeks prior to the first dose of NKX019
12. Active CNS SLE . 13. Active bleeding disorders.
- - Any overlapping autoimmune condition for which the condition or the treatment of the
condition may affect the study assessments or outcomes (e.g. scleroderma with
significant pulmonary hypertension; any condition for which additional
immunosuppression is indicated).
Overlapping conditions for which the condition or
treatment is not expected to affect assessments or outcomes (e.g. Sjögren's
syndrome) are not excluded.
- - Pregnancy, breast feeding or, if of childbearing potential, not using adequate
contraceptive precautions or planned oocyte and sperm donation.
- - Current/active infection, and any infection requiring systemic antimicrobial therapy
within the past 30 days of planned LD.
- - History of positive HIV antibody or test positive at screening.
Hepatitis B or C
positive at screening, active tuberculosis (TB) or latent TB requiring suppressive
therapy.
- - Major surgery within 28 days prior to the first dose of NKX019 or any surgery from
which the subject has not recovered or has ongoing complications.
- - Malignancy within 5 years of screening, with the exception of basal and squamous
cell carcinomas treated by complete excision.
Subjects with cervical dysplasia that
is cervical intraepithelial neoplasia but have been treated with conization or loop
electrosurgical excision procedure, and have had a normal repeat Papanicolaou test
are allowed.
- - Prior cellular therapy, including mesenchymal, CAR-T or CAR-NK cells.
- - Prior cerebrovascular ischemia/hemorrhage including transient ischemic attack within
90 days prior to the first dose of NKX019.
- - Any other acute or chronic medical or psychiatric condition, or known laboratory
abnormality that, in the Investigator's opinion is expected to:
1.
Increase the risk associated with study participation or NKX019 administration. 2. Interfere with the informed consent process, compliance with the study
requirements, or interpretation of the study results. 3. Make the subject inappropriate for entry into this study. 4. Require concomitant use of any medication that is listed as prohibited while on
study. 5. In the opinion of the Investigator, clinically significant drug or alcohol
abuse within 2 years prior to screening.
- - Prior therapies for SLE, including investigational agents, within 4 weeks or 5
half-lives of the drug (whichever is longer) prior to lymphodepletion.
- - Currently taking or known need for any of the medications prohibited in the study
protocol.
- - Known hypersensitivity or contraindications to the study treatment including LD; or
other components such as human serum albumin or dimethyl sulfoxide.
