- - Male or female ≥21 years and ≤60 years old and with BMI ≤40
- Moderate to severe knee OA pain ≥3 and ≤8 on the NRS pain subscale in the affected
knee (signal knee)
- Mild or no pain ≤3 on the numeric rating scale (NRS) pain subscale in the
contralateral knee (unaffected knee)
- Persistent knee pain lasting at least 3 months prior to Screening
- Active life-style (play or train themselves at least 2 to 3 times per week)
- Diagnosis of knee OA confirmed by radiographic assessment (must be performed within 6
months prior to screening visit):
anterior-posterior view (weight bearing extension or semi-flexion) of
tibiofemoral joint, (posterior-anterior/Rosenberg view as an additional
2. lateral view of both the tibiofemoral and patellofemoral joints
3. Merchant X-ray (or Sunrise) view for patellofemoral joint.
- - Radiographic assessment must confirm K-L Grade 1 to 3 for TFOA and/or K-L Grade 1 to
- - Radiographic assessment confirms normal articulation of patella and trochlea in
Merchant (or Sunrise) view
- Radiographic assessment confirms abnormal patellofemoral tracking or articulation in
lateral and/or Merchant view (or Sunrise View)
- Major injury to or disorder of the contralateral knee or other weight-bearing joint
that would interfere with the study assessments.
- - Secondary OA of the study joint due to a prior or concomitant condition (e.g., septic
arthritis, inflammatory joint disease, gout, articular fracture, major dysplasia, or
congenital abnormality, hemochromatosis, etc.).
- - Surgery to the study joint within the previous 12 months prior to Screening.
to the contralateral knee or other weight-bearing joint within the previous 12 months
that would interfere with the study assessments.
- - Patients with either total joint replacement implants, unicondylar implants or
patellofemoral replacement implants in the affected joint.
- - Ligament reconstruction of the affected knee within 3 years.
- - Inflammatory arthropathies such as rheumatoid arthritis, lupus, or psoriatic
- - Episode of gout or calcium pyrophosphate (pseudogout) diseases within 6 months prior
- - IA or local peri-articular corticosteroid injections to the study joint/knee within 8
weeks (2 months) prior to Screening or to any other joint (other than the study joint)
or soft tissue area within 1 month prior to Screening.
- - Any oral corticosteroid within 1 month prior to Screening.
Steroid inhalants are
permitted if the Subject has been on a stable regimen for the past 1 month prior to
Screening and remains on this regimen throughout the course of the trial.
- - Intra-articular HA in the study joint within 6 months prior to Screening.
- - History of allergic reaction to an intra-articular Hyaluronic acid injection.
- - Known hypersensitivity (allergy) to gram positive bacterial proteins.
- - Use of glucosamine or chondroitin sulfate containing products unless the subject is on
stable doses for at least 14 days prior to Screening and willing to remain on these
stable doses throughout the course of the study.
- - Inability to perform the climbing stair test.
- - X-ray findings of acute fractures, severe loss of bone density, avascular necrosis
and/or severe deformity.
- - Axial deviation of the lower limbs greater than 20 degrees in valgus or varus on
- - Symptomatic OA of either hip or spine with a pain score ≥2 on the NRS on or off pain
- - Clinically significant medio-lateral and/or anterior-posterior instability.
- - Osteonecrosis of either knee.
- - Participation in a physical therapy regimen that has not been stable during the 1
month prior to Screening and the subject is unwilling or unable to maintain the same
regimen throughout the course of the trial or is unwilling to stop previously
prescribed or self-initiated physical therapy regimen and switch solely to and
initiate study defined PEP program.
- - K-L Grade 4 TFOA of the knee (i.e., large osteophytes, marked narrowing, severe
sclerosis, and definite deformity).
- - K-L Grade 4 PFOA of the knee (i.e., large osteophytes, marked narrowing, severe
sclerosis, definite deformity)
- Hemiparesis of the lower limbs.
- - Significant surgery of lower limbs (hip, ankle, foot) that may interfere with knee
- - Chronic use of analgesia for pain (including pain in the other knee or any other
joint) that may interfere with the evaluations of the test knee (such as possible use
of rescue medication for these other conditions).
- - Known allergies to acetaminophen and hyaluronan preparations.
- - Know hypersensitivity (allergy) to anesthetics
- Recurrent medical history of severe allergic or immune-mediated reactions.
- - Active infection or skin diseases in the area of the potential injection site or
- - Any dermatological disease overlying the signal joint that would contraindicate
multiple injections or aspirations.
- - Use of any agent reported to have symptom relief for arthritis or be a
disease/structure modifying drug (e.g. doxycycline, long-term tetracycline,
s-adenosylmethionine [SAM], methylsulfonylmethane [MSM] or dimethylsulfoxide [DMSO],
dietary supplements or any herbal remedy taken for arthritic and joint conditions
within the past month (exceptions as specified in Exclusion #14).
- - Peripheral neuropathy that would be severe enough to interfere with the evaluation of
- - Psychological status (e.g., anxiety, depression, poor sleep quality, pain
catastrophizing, etc.) that may interfere with functional assessment of the target
- - Vascular insufficiency of lower limbs that is severe enough to interfere with the
evaluation of the subject.
- - Concomitant therapy with anticoagulants (low dose aspirin, not exceeding 325 mg per
day as an anti-thrombotic agent is permitted if stable for 1 month prior to Screening
and remains stable throughout the study).
- - Any concomitant disease(s) or condition(s) that may interfere with the free use and
evaluation of the affected knee for the 6 months course of the trial (cancer, other
rheumatic diseases, gout, severe congenital defects, etc.).
- - Any serious mobility problems (e.g. Parkinson's disease) or claustrophobia
- Any concomitant chronic disease(s) or condition(s) that may predispose them to a high
probability of interfering with the completion of the 6-month follow-up of the study
such as peptic ulcer, liver disease, severe coronary disease, renal disease, cancer,
pregnancy, alcoholism, drug abuse, mental state, or other clinically significant
- - Excessive alcohol consumption or alcoholism that would be contraindicated with the use
- - Use of marijuana, whether prescribed or not
- Any clinically significant diagnostic test and/or abnormal laboratory test result(s)
that, in the opinion of the clinical investigator, may place the subject's health at
risk, impact the study, or impact the subject's ability to complete the study.
- - Inability to legally comprehend the details and nature of the study for any reason,
including psychiatric illness.
- - Likelihood of protocol violations or unlikely to compete the study for any reason, as
determined by the clinical investigator.
- - Continued participation in an experimental drug/device study or any clinical trial
within the previous 8 weeks prior to Screening.
Subjects must have fully completed
participation in an experimental drug/device study of any clinical trial at least 8
weeks prior to screening.
- - Pregnancy, breastfeeding, planned conception, premenopausal women who have not had
tubal ligation, hysterectomy, or are unwilling or unable to utilize contraceptive
measures (or contraception)
- Prior history of any malignancy with the exception of basal cell carcinoma of the skin
treated more than 2 years prior to Screening.
- - Significant bleeding diathesis.
- - Participation in current litigation for injuries related to the study knee or other
injuries that might interfere with their completion of the study protocol.
- - Non-English speaking subjects.